A-A-A leverages F-actin expression in middle-aged female fibroblasts to level of young fibroblasts
WEDNESDAY, July 15, 2015 (HealthDay News) — New research published online July 3 in the International Journal of Cosmetic Science has characterized the anti-aging potential of acetyl aspartic acid (A-A-A).
Johanna Gillbro, Ph.D., from Oriflame Skin Research in Stockholm, and colleagues used structural activity relationship (SAR) analysis to identify a predictive mechanism of action of A-A-A. Wound healing potential was assessed in vitro using scratch motility tests with immortalized keratinocytes. Multiplex protein assays were used to analyze matrix metalloproteinase 1-3 (MMP 1-3), and polymerized actin was stained in human dermal fibroblasts (HDF).
Based on SAR analysis, the researchers predicted that A-A-A would possess epidermal and dermal activities, with wound healing and MMP-inhibition potential detected. Wound healing potential was confirmed using keratinocyte scratch motility assays. The dermal activities predicted by A-A-A inhibition of MMP 1-3 in HDF were confirmed. There was a positive association between age and F-actin. Seventy-two hour stimulation of HDF with A-A-A reduced the polymerized cytoskeletal network, as seen by F-actin expression inhibition. In middle-aged female fibroblasts (50 years), A-A-A leveraged F-actin expression to the level of young female fibroblasts (30 years), corresponding to a 40 percent decrease in F-actin expression.
“Using an in silico and in vitro approach, we were able to demonstrate that A-A-A has the capacity to target different compartments of the skin through keratinocyte regeneration, MMP-inhibition, and relief in fibroblasts stiffness by reduction of F-actin cytoskeletal network in HDF,” the authors write.
The study was funded by Oriflame Cosmetics AB.
Abstract
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